Cat. No.
MABL-3134
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Rhesus Monkey, Cynomolgus Monkey
Clone No.
14F128
From
Recombinant Antibody
Specificity
This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production.
Alternative Names
TREM1; CD354; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1
UniProt
Q9NP99
Immunogen
The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.
Application Notes
The original IgG1 antibody binds human TREM-1 with an affinity of 2.4 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was able to reduce activation of BWZ/hTREM-1 reporter cell. This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
