Cat. No.
MABL-3136
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
T9
From
Recombinant Antibody
Specificity
This antibody binds Triclocarban (TCC), which is an antibacterial chemical. This antibody also shows some cross reactivity with structurally similar compounds like 2′-OH TCC and 4,4′-Dichlorocarbanilide.
Alternative Names
TCC; 1-(4-chlorophenyl)-3-(3;4-dichlorophenyl)urea; bactericide
UniProt
Immunogen
The original antibody was generated by immunizing Llama glama intramuscularly with 600 μg of the TCC-Thy conjugate in Freund Incomplete Adjuvant.
Application Notes
The binding of this antibody to triclocarban was confirmed using ELISA. SPR was used to measure the binding affinity of this antibody and it was reported to be Kd= 1.1 nM.
Antibody First Published
Rosa et al. Competitive Selection from Single Domain Antibody Libraries Allows Isolation of High-Affinity Antihapten Antibodies That Are Not Favored in the llama Immune Response. Anal Chem. 2011 Sep 15; 83(18): 7213–7220. PMID:21827167
Note on publication
Describes the isolation of high affinity nanobodies against Triclocarban.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
