Cat. No.
MABL-3158
Application
Blocking, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
MTW-1
From
Recombinant Antibody
Specificity
This antibody is specific for murine TWEAK. It has been shown not to react with TNF-α, FasL, and TRAIL.
Alternative Names
CD255; TNFSF12; TNF-related weak inducer of apoptosis; MTW1; MTW 1; Tumor necrosis factor ligand superfamily member 12;
UniProt
O54907
Immunogen
This antibody was raised by immunising SD rats with CD8-mTWEAK, a fusion of the human CD8alpha extracellular region to murine TWEAK.
Application Notes
The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).
Antibody First Published
Nakayama et al. Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies. Biochem Biophys Res Commun. 2003 Jul 11;306(4):819-25. PMID:12821115
Note on publication
Describes the original generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
