Cat. No.
MABL-3179
Application
in vitro neutralization, ELISA
Isotype
Engineer antibody
Species Reactivity
Vaccinia virus
Clone No.
SC212
From
Recombinant Antibody
Specificity
The antibody is specific for VACV.
Alternative Names
VV
UniProt
Immunogen
A phage display library of human single chain variable fragment (scFv) antibodies was constructed from the B cells of healthy volunteers boosted by the VACV vaccine. Human scFv was isolated by panning against VACV.
Application Notes
The scFv fragment was converted to human IgG1 (VC212). The binding affinity of VC212 was estimated by competition ELISA to IC50 values 22 µg/ml (146.67 nM). The humanized version of the antibody neutralized the VACV, as evidenced by the plaque reduction neutralization test result with a PRNT50 of ~0.16 mg/ml (~1.07 µM) (Shin et al., 2019; PMID: 30856707).
Antibody First Published
Shin et al. Selection of Vaccinia Virus-Neutralizing Antibody from a Phage- Display Human-Antibody Library J Microbiol Biotechnol. 2019 Apr 28;29(4):651-657. PMID:30856707
Note on publication
The original paper describes the generation and characterization of the antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
