Cat. No.
MABL-3194
Application
IP, WB, Block, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
VD1 (4A5)
From
Recombinant Antibody
Specificity
This antibody binds human VEGF-D and block the binding of VEGF-D to both VEGFR-2 and VEGFR-3. Peptide-based mapping and site-directed mutagenesis suggested that binding epitope of this antibody includes the five residues 147NEESL151of the VEGF-D (PMID: 21315726). VEGF-D signaling through the VEGFR-3 receptor has been shown to be the primary source of lymphangiogenic activation and has also been noted in pathogenic angiogenesis in some tumors.
Alternative Names
VEGFD; FIGF; Vascular endothelial growth factor D; c-Fos-induced growth factor
UniProt
O43915
Immunogen
The original antibody was generated by immunizing female BALB/c mice with truncated portion of human VEGF-D from residue 93 to 201, which had FLAG-tag at its N-terminus.
Application Notes
The original mouse antibody VD1 competes with VEGF-D to bind both VEGFR-2 and VEGFR-3 . VD1 blocks the mitogenic response of human microvascular endothelial cells to VEGF-D. The binding specificity of the original mouse version of this antibody towards human VEGF-D was determined using ELISA. The original mouse antibody was capable of immunoprecipitating human VEGF-D. This antibody was also capable of detecting VEGF-D in a western blot assay (PMID: 10785369). This antibody was used in a study involving a mouse tumor model to explore the role of VEGF-D in promoting both angiogenesis and lymphangiogenesis in tumors (PMID: 11175849). This antibody was also used to identify the role of VEGF-D in promoting tumor growth and spread though lymphatic system in a mouse model of hepatocellular carcinoma (PMID: 18338756). This antibody was used to study the localization of VEGF-D in primary tumors by immunohistochemical analysis (PMID: 12148568).
Antibody First Published
Achen et al. Monoclonal antibodies to vascular endothelial growth factor-D block its interactions with both VEGF receptor-2 and VEGF receptor-3. Eur J Biochem. 2000 May;267(9):2505-15. PMID:10785369
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
