Key features and details | |
Cat. No. | MABL-1064 |
Name | Anti-CD63 mAbs |
Clone No. | AFD- NK-1-C3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades. |
Alternative Name | Granulophysin; LAMP-3; Tetraspanin-30; Tspan-30; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; CD63 antigen; NK1/C3; NKI/C3; NK-1.C3; NK1C3 |
UniProt | P08962 |
Immunogen | This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells. |
Application Notes | The antibody NK-1-C3 has been widely used for the detection and analysis of CD63 via various methods. For instance, NK-1-C3 (NK1/C3) proved successfull in labelling CD63 in multiple tissues, for example, when localizing CD63 in murine retinal pigment epithelium (RPE)/choroid tissue (Wang et al., 2009). Similarly, this antibody was used to detect CD63 on melanoma cells via immunofluorescence staining (Khazen et al., 2016). |
Antibody First Published | Vennegoor et al. Biochemical characterization and cellular localization of a formalin-resistant melanoma-associated antigen reacting with monoclonal antibody NKI/C-3. Int J Cancer. 1985 Mar 15;35(3)11:45287-95. PMID:3882581 |
Note on publication | This article describes the generation and characterization of this anntibody (NKI/C-3). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
