Key features and details | |
Cat. No. | MABL-1065 |
Name | Anti-CD64 mAbs |
Clone No. | AFD-AT152-9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds mouse Fc gamma RI, which is a high affinity receptor for the Fc region of immunoglobulins gamma. It functions in both adaptive and innate immune responses |
Alternative Name | FcgRI; Fcgr1; FcRI; FcγRI; FcgammaRI; Fc-gamma RI; IgG Fc receptor I; High affinity immunoglobulin gamma Fc receptor I |
UniProt | P26151 |
Immunogen | |
Application Notes | This antibody binds 293F cells transfected with mFcγRIV with a binding affinity of 4.3nM. This antibody could positively stain mFcγRI expressed on the surface of macrophages and monocytes in a flow cytometry (PMID: 26512139). The plate-coated antibody could also detect mFcγRI-rCD4 fusion proteins in an ELISA (PMID: 26512139). This antibody was used for aggregation of FcgRI on BM-DCs and peritoneal MF cells for microscopy assays (PMID: 37360697). This antibody was used for the immunofluorescence staining of mouse lung tissues (PMID: 38690277). |
Antibody First Published | Tutt et al. Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors. J Immunol. 2015 Dec 1;195(11):5503-16. PMID:26512139 |
Note on publication | This paper describes the generation and characterization of antibodies against mouse and human FcY receptors. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
