Cat. No.
MABL-3204
Application
functional assay, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
V1
From
Recombinant Antibody
Specificity
This antibody is specific for the VEGFR2 protein. It shows no binding to individual domains 2-3, 1-3 and 1-4. VEGFR2 is a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. It plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells.
Alternative Names
Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; CD309
UniProt
P35968
Immunogen
The original human version of this antibody was created by screening a human phage display library with VEGFR2-Fc.
Application Notes
The human version of this antibody was tested by ELISA with different domains of VEGFR2 (WO2017004254). Furthermore, binding of the human version of this antibody to human umbilical vein endothelial cells was demostrated using flow cytometry (WO2017004254). After that, HUVEC tube formation assays were preformed using the human version of this antibody. This antibody did not show significant inhibition of tube formation (WO2017004254). Further, a matrigel plug assay was preformed using the human version of this antibody to asses its ability to inhibit angiogenesis in vivo (WO2017004254).
Antibody First Published
Wei-dong et al. Anti-vascular endothelial growth factor receptor 2 (vegfr2) antibodies WO2017004254
Note on publication
Describes the generation and characterization of this antibdy.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
