Cat. No.
MABL-3219
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Chinese rare minnow, common carp, crucian carp, Zebrafish
Clone No.
B5
From
Recombinant Antibody
Specificity
B5 recognises a linear epitope of Vtg at the N-terminal region of the protein from zebrafish, Chinese rare minnow, common carp and crucian carp. Vtg is the phospholipoglycoprotein precursor to the major egg yolk protein which is synthesized in the liver in response to endogenous estrogen in females. Vtg synthesis in males can be used as a biomarker for exposure to estrogen and estrogen mimics in males where Vtg levels are normally low but can be increased upon exposure.
Alternative Names
Vtg
UniProt
Q90YN8
Immunogen
B5 was isolated from a phage display library constructed using spleenic mRNA from non- immunized mice after 3 rounds of panning.
Application Notes
B5 scFv can be used in WB and ELISA studies.
Antibody First Published
Rao et al. Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species Dis Aquat Organ. 2010 Dec 7;93(1):83-91. PMID:21290899
Note on publication
Describes the production of B5 scFv a from phage display library and the characterisation of the scFv by ELISA and WB to determine binding specificity and binding curves. A 152aa N-terminal protein fragment from Vtg from different cyprinid fish species was used in this study.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
