Key features and details | |
Cat. No. | MABL-1091 |
Name | Anti-CD71 mAbs |
Clone No. | AFD- OKT9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, functional assay, WB, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the Transferrin receptor protein 1. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. |
Alternative Name | Transferrin receptor protein 1; TFRC; TR; TfR; TfR1; Trfr; T9; p90 |
UniProt | P02786 |
Immunogen | This antibody was raised by immunizing CAF mice with human thymocytes. |
Application Notes | Lysis of Lymphoid Populations was tested with this antibody and rabbit complement (Reinherz et al, 1980; pmid:6966400). Human thymocytes and peripheral T cells were analyzed using flow cytometry with this antibody (Reinherz et al, 1980; pmid:6966400). This antibody is able to deplete the A24- binding protein from U937 cell lysates (Moura et al, 2001; pmid:11514599). Tumor cells from lymph node or bone marrow aspirate or homogenized tumor sample were used in a flow cytometry experiment with this antibody (Feremans et al, 1991; pmid:1996560). A double labeling with this antibody and phycoerythrin- conjugated anti-CD 19 was preformed on a human patient (Feremans et al, 1991; pmid:1996560). This antibody was used for immunoblotting on Fibrosarcoma cell line HT1080 and epidermoid carcinoma line A431 (Claas et al, 2001; pmid:11113129). Cells from heparinized bone marrow were used for flow cytometry using this antibody (Krystal et al, 1994). |
Antibody First Published | Reinherz et al. Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588-92. PMID:6966400 |
Note on publication | A series of monoclonal antibodies was used to define three discrete stages of human intrathymic T-cell differentiation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
