Key features and details | |
Cat. No. | MABL-1092 |
Name | Anti-CD71 mAbs |
Clone No. | AFD- OX-26 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, WB, FC, IF, IHC-Fr |
Species Reactivity | Rat, Human |
Basic Information | |
Specificity | This antibody is specific for the rat transferrin receptor, also known as CD71. |
Alternative Name | transferrin receptor; TfR; p90; T9; TFR1; OX26; MRC OX26; MRC OX-26 |
UniProt | Q99376 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with phytohaemagglutinin (PHA)-activated PVG rat lymph node cells with NS 1/1.Ag 4.1 myeloma cells. |
Application Notes | The original format of this antibody (mouse IgG2a) was developed and demonstrated to effectively labele transferrin receptor-expressing cells, including dividing lymphocytes and erythroid precursors, with minimal binding to non-dividing lymphoid cells or mature erythrocytes. It was used for fluorescence-based cell sorting to isolate subpopulations of rat hematopoietic stem and progenitor cells from bone marrow and fetal liver (Jefferies et al., 1985; PMID: 2981766). The original format of the antibody was employed in immunohistochemical staining of cryostat sections from rat brain. In vivo testing in PVG/c rats involved injecting the antibody intravenously, followed by cryostat sectioning and staining. The results showed clear labelling of rat brain capillaries, especially in the cerebellum and cortex, with weaker labelling after 16 hours. No such labelling was observed in other rat tissues, supporting the specificity for brain capillaries (Jefferies et al., 1984; PMID: 6095085). |
Antibody First Published | Jefferies et al. Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor. Immunology. 1985 Feb;54(2):333-41. PMID:2981766 |
Note on publication | The original publication describes the generation and examines the specificity and binding characteristics of the mouse monoclonal antibody MRC OX-26 against the rat transferrin receptor. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
