Key features and details | |
Cat. No. | MABL-1099 |
Name | Anti-CD73 mAbs |
Clone No. | AFD-1E 9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, IP, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)- linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73. |
Alternative Name | NT5E; 5'-NT; 5'-nucleotidase; Ecto-5'-nucleotidase |
UniProt | P21589 |
Immunogen | The original antibody was generated by immunizing mice with highly purified human placental 5'-NT as immunogen. |
Application Notes | The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody inhibits soluble placental 5'-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. This antibody was also used in the study of cellular distribution of CD73 (PMID: 2137649). It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab')2 goat anti-mouse Ig (PMID: 2137649, 2550543). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). This antibody was also used to detect CD73 in COS cells transfected with CD73 cDNA using flow cytometry (PMID: 7595232). |
Antibody First Published | Thomson et al. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). Tissue Antigens (1990); 35(1):9-19. PMID:2137649 |
Note on publication | Describes the generation and characterization of this antibody and its use in studying the expression of CD73 on various types of blood lymphocytes. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
