Key features and details | |
Cat. No. | MABL-1100 |
Name | Anti-CD73 mAbs |
Clone No. | AFD- AD2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)- linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73. |
Alternative Name | NT5E; 5'-NT; 5'-nucleotidase; Ecto-5'-nucleotidase |
UniProt | P21589 |
Immunogen | The original antibody was generated by immunizing mice with full-length CD73 purified from human placenta. |
Application Notes | The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody promotes cell-surface clustering of CD73 followed by internalization, thus inhibiting the ability of circulating tumor cells to extravasate and colonize, leading to inhibition of metastasis. This antibody inhibited development of metastasis in a spontaneous animal model of human metastatic breast cancer. Flow cytometry analysis showed that anti-CD73 AD2 mAb (AD2 mAb) recognized CD73 on the human breast cancer cell lines LM3 and MDA-MB-231, but failed to detect the murine CD73 variant expressed on isolated mouse mononuclear hematopoietic cells(PMID: 24043904). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
