Key features and details | |
Cat. No. | MABL-1101 |
Name | Anti-CD74 mAbs |
Clone No. | AFD- BU45 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, IP, RIA, WB, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the lumenal portion of human CD74. |
Alternative Name | li; Ia antigen-associated invariant chain; HLA-DR antigens-associated invariant chain; HLA class II histocompatibility antigen gamma chain; invariant chain |
UniProt | P04233 |
Immunogen | The original antibody was raised by immunizing a mouse with B lymphoblastoid cell line, HFBl. |
Application Notes | The original version of this antibody was used for flow cytometry on single cell suspensions from the spleen (Genève et al., 2014; PMID:25171001), and on (Wraight et al., 1990; PMID:1690714), and on gastric epithelial cells (Barrera et al., 2005; PMID:15923369). This antibody was used for immunofluorescence on transfected human fibroblast cell lines (M1DRIi) (Stang and Bakke., 1997; PMID:9281355), on Stably transfected MDCK cells (Simonsen et al., 1998; PMID:9650780) and on B cells (Roche et al., 1993; PMID:8397411). Furthermore, the antibody was used for immunoprecipitation on class Il-negative B x T hybrid cell line T2 and its class II positive parental cell line T1 (Henne et al., 1995; PMID:7750992), on U937 cell lysates (Wraight et al., 1990; PMID:1690714), on stably transfected MDCK cells grown (Simonsen et al., 1998; PMID:9650780), and on gastric epithelial cells (Barrera et al., 2005; PMID:15923369). |
Antibody First Published | Wraight et al. Human major histocompatibility complex class II invariant chain is expressed on the cell surface Biol Chem. 1990 Apr 5;265(10):5787-92. PMID:1690714 |
Note on publication | The original paper describe a study with three monoclonal antibodies which recognize an extracytoplasmic determinant present on all forms of the invariant chain and use them to demonstrate its presence on the surface of the intact cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
