Key features and details | |
Cat. No. | MABL-1114 |
Name | Anti-CD8 mAbs |
Clone No. | AFD-14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, IP, WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | Clone 14 binds human CD8, which is a coreceptor for the T lymphocyte receptor (TCR). CD8 binds, as a heterodimer, to class 1 MHC molecules and facilitates antigen-specific activation of the adaptive immune response. CD8 mutations are associated with various immunodeficiencies and pathologies. |
Alternative Name | Leu2; cluster of Differentiation 8; T lymphocyte differentiation antigen T8 |
UniProt | |
Immunogen | Murine antibodies were produced by immunising mice with human monocytes. Immunised cells were used to produce a hybridoma from which the antibodies were purified. |
Application Notes | Clone 14 has been used to investigate the cell type-dependent expression of CD8 in nephrectomised rats by IHC methods (Xiao et al, 2014), and can further be used for detection of T cell population subsets. |
Antibody First Published | Hogg et al. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional β2 integrins Mac-1 and LFA-1 J. Clin. Invest. 103:97–106 (1999). PMID:9884339 |
Note on publication | Describes a patient with leukocyte adhesion deficiency 1 (LAD-1) syndrome, characterised by lack of CD18 function. In this study, mAb clone 14 - produced in the authors's laboratory - was used as an anti-CD8 marker. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
