Key features and details | |
Cat. No. | MABL-1115 |
Name | Anti-CD8 mAbs |
Clone No. | AFD-51.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CD8. |
Alternative Name | CD8a; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2 |
UniProt | P01732 |
Immunogen | The original antibody was generated by immunizing mice with CD1d. |
Application Notes | The antibody could block HLA-A2.1-mediated binding to CD8α(+) expressed on CHO cells (Norment et al, 1988; PMID: 3263576). The antibody could also block TLA-mediated binding (Teitell et al., 1991; PMID: 1834760). The antibody was used to localize CD8α to the cell periphery on monocytes using confocal microscopy of permeabilized PBMC. The antibody could detect CD8α by flow cytometry on CD14hi monocytes from human peripheral blood (Gibbings et al. 2007; PMID: 17678538). |
Antibody First Published | Martin et al. Cell-cell adhesion mediated by CD8 and MHC class I moleculesEpitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32 J Immunol. 1984 Feb;132(2):759-65. PMID:6197474 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
