Key features and details | |
Cat. No. | MABL-1127 |
Name | Anti-CD80 mAbs |
Clone No. | AFD- #112 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP;IF;IHC, FC |
Species Reactivity | Chicken |
Basic Information | |
Specificity | This antibody is specific for chicken CD80, a type I glycoprotein consisting of two extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail. Functionally, CD80 on antigen presenting cells (APCs) binds to CD28 on T cells, thereby providing a costimulatory signal necessary for T cell activation. |
Alternative Name | Cluster of Differentiation 80; CD80 protein; chicken CD80; chCD80 |
UniProt | Q5F3J1 |
Immunogen | The original antibody was raised by immunizing BALB/c mice with purified chicken CD80. |
Application Notes | This antibody was used for detection of chicken CD80 expressed on CHO cells by flow cytometry; it was also used to detect CD80 on monocytes in the bursa of Fabricius and on spleen monocytes. chCD80 from transfected CHO cells, or from LPS-stimulated mononuclear lymphocytes were used for immunoprecipitation. In this procedure the protein was immunoprecipitated using this antibody.This antibody was used for immunohistochemistry on the bursa of Fabricius and spleen. Immunofluorescence was performed on chicken intestinal dendritic cells using this antibody. The antibody inhibited ConA-stimulated spleen cell differentiation into MHCII+ cells and blocked IL-2 induced spleen blast cell proliferation (Lee et al, 2011; pmid: 21334748). |
Antibody First Published | Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken CD80. Comp Immunol Microbiol Infect Dis. 2011;34(3):273‐279 PMID:21334748 |
Note on publication | The paper describes the generation and characterization of the antibody, which is specific for chicken CD80. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
