Key features and details | |
Cat. No. | MABL-1140 |
Name | Anti-CD86 mAbs |
Clone No. | AFD- 3D1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, FC |
Species Reactivity | Pig, Human |
Basic Information | |
Specificity | This antibody binds human B7-2 (CD86). |
Alternative Name | B7-2; B7.2; B70; BU63; CD28LG2; FUN-1; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; CTLA-4 counter-receptor B7.2; CTLA4L; CTLA-4L |
UniProt | P42081 |
Immunogen | The original antibody was generated by immunizing mice with human B7-2. |
Application Notes | The original human IgG1, 3D1 antibody binds human B7-2 with an affinity of approximately Kd= 1nM and can be used to identify human B7-2 in an ELISA. This antibody was capable of immunoprecipitating B7-2 exrpressing porcine microvascular endothelial cells (PMID： 8757955). This antibody was capable of identifying CHO cells transfected with B7-2 in a flow cytometric assay. |
Antibody First Published | Davis et al. Primary porcine endothelial cells express membrane-bound B7-2 (CD86) and a soluble factor that co-stimulate cyclosporin A-resistant and CD28-dependent human T cell proliferation. Int Immunol. (1996); 8(7)：1099-111. PMID：8757955 |
Note on publication | Describes the use of this antibody for detection of B7-2. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
