Key features and details | |
Cat. No. | MABL-1149 |
Name | Anti-CD8a mAbs |
Clone No. | AFD- VHHCD8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody is specific for CD8. |
Alternative Name | Lyt-2; Lyt2; VHH-X118; T-cell surface glycoprotein Lyt-2 ; T-cell surface glycoprotein CD8 alpha chain |
UniProt | P01731 |
Immunogen | The original antibody was generated by immunizing a llama with CD8, followed by antibody library construction. The antibody was isolated by biopanning against CD8. |
Application Notes | The binding affinity of the original format of the antibody to CD8 was measured by surface plasmon resonance (Kd= 0.9 nM). FACS analysis of splenocytes and lymp nodes gated on CD45 + CD19- CD3+ cells, confirming that the original format of the antibody stained CD8+ cells. The antibody competed with a commercial anti-CD8α antibody, showing it bound to the CD8α chain. 89Zr- PEGylated antibody was used for PET imaging on mice, to track the presence of intratumoral CD8+ T cells (Rashidian et al., 2017; PMID: 28666979). |
Antibody First Published | Rashidian et al. Predicting the response to CTLA-4 blockade by longitudinal noninvasive monitoring of CD8 T cells J Exp Med. 2017 Aug 7; 214(8)： 2243–2255. doi： 10.1084/jem.20161950 PMID：28666979 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
