Key features and details | |
Cat. No. | MABL-1150 |
Name | Anti-CD8A mAbs |
Clone No. | AFD- YCATE55.9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, cell-mediated lympholysis assays., fixed lymphocyte reaction (MLR), FC, IF, IHC-Fr |
Species Reactivity | Dog |
Basic Information | |
Specificity | This antibody is specific for the alpha chain of canine CD8, and reacts with a rat cell line transfected with cDNA for canine CD8α (Gorman, 1994) |
Alternative Name | LEU2; T-cell surface glycoprotein CD8 alpha chain; CD8a |
UniProt | P33706 |
Immunogen | This antibody was prepared by immunizing rats with canine leucocytes, thymocytes or concanavalin A-activated lymphoblasts. |
Application Notes | This antibody recognizes canine CD8α, and has been used in in IF and IHC studies to establish that CD8 expression is confined to a minority population of T cells, as is the case for other species (Cobbold, 1994). In a canine transplant model, administration of this antibody, in combination with another anti-CD8 antibody (YCATE60.3.9) causes a 90 per cent depletion of CD8+ T cells from the peripheral blood, but does not result in prolonged allograft survival (Watson, 1993). However, the administration of this antibody in combination with an anti-CD4 antibody is associated with an immunosuppressive effect, and an extended median graft survival time. This antibody blocks MHC class I dependant T-cell responses in vitro and in vivo. |
Antibody First Published | Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens： Summary of the First International Canine Leukocyte Antigen Workshop (CLAW) Tissue Antigens. 1994 Mar;43(3)：137-54. PMID：8091414 |
Note on publication | Describes the identification and characterisation of a panel of monoclonal antibodies that define canine homologues of human CD antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
