Key features and details | |
Cat. No. | MABL-1161 |
Name | Anti-CD96 mAbs |
Clone No. | AFD- BL006-19-14G3-14K1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, SPR, ELISA, FC |
Species Reactivity | Human, Rhesus Monkey |
Basic Information | |
Specificity | This antibody is specific for the D2 domain of the shorter form of human CD96 (isoform v2). It was found to also bind the long for of human CD96, albeit with lower affinity, and to cross-react with Rhesus CD96. |
Alternative Name | Tactile; T cell activation, increased late expression; Cluster of Differentiation 96 |
UniProt | P40200 |
Immunogen | The original antibody was generated by immunizing wild-type mice with human CD96 DNA, followed by a boost with CHO cells transfected with the human CD96 expression vector. |
Application Notes | The original antibody (Mouse IgG2a) was selected using ELISA and flow cytometry to confirm binding to CD96. FACS testing showed that this antibody exhibits strong binding to the human CD96 short isoform (CD96v2) expressed on CHO cells (EC50 = 3.10 nM) and strong but weaker binding to the human CD96 long isoform (CD96v1). This antibody showed partial inhibition of the CD96/CD155 interaction, though it did not achieve 50% inhibition at the maximum tested concentration (30 μg/mL, 200 nM), with a relative IC50 calculated at 2.67 nM. FC analysis revealed strong binding to T, NK, and NKT cells from human peripheral blood mononuclear cells (PBMCs). SPR measurement revealed a KD of 0.254 nM towards an in- house produced hCD96-hFc protein. The antibody demonstrated strong co-stimulatory activity by enhancing T cell activation and proliferation in the presence of suboptimal anti-CD3 stimulation, effectively increasing CD25 expression and CFSE dilution in CD4+ and CD8+ T cells, with Fc receptor cross-linking being required for its activity. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
