Key features and details | |
Cat. No. | MABL-1160 |
Name | Anti-CD96 mAbs |
Clone No. | AFD- BA143 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | activation, functional assays, in vitro, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for Human CD96 and does not cross-react with Monkey CD96. |
Alternative Name | Tactile; T cell activation, increased late expression; Cluster of Differentiation 96 |
UniProt | P40200 |
Immunogen | The original antibody was generated by immunizing mice with human CD96. |
Application Notes | The original antibody (Mouse IgG1) demonstrated high-affinity binding to CHO cells expressing human CD96 isoform 2 (EC50 = 5.9 ng/mL), as validated in an ELISA. It effectively blocked CD155-Fc binding to the same target (IC50 = 152.4 ng/mL) in a competitive binding assay. It also exhibited binding to CHO cells expressing the V/V variant of human FcγRIIIa (EC50 = 191.5 ng/mL), confirmed through an ELISA. In an in vitro T cell activation assay, the antibody successfully bound to activated CD4+ and CD8+ T cells from three donors, and in a staphylococcal enterotoxin A (SEA)-stimulation assay, it showed limited functional activity in promoting IL-2 secretion by PBMCs. Additionally, it displayed a binding affinity (KD = 13.3 nM) for the full-length extracellular domain of human CD96 in a kinetic analysis (US20240141039A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
