Anti-CD96 mAbs

Cat. No.

MABL-1163

Name

Anti-CD96 mAbs

Clone No.

AFD- ch1720

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, in vitro, SPR, ELISA

Species Reactivity

Cynomolgus Macaque, Human

Basic Information

Specificity

This antibody is specific for Human CD96 but   cross-reacts with Monkey CD96. It exhibits strong binding to the peptide   sequence IAVYHPQYGFYCAYGRPCES in CD96.

Alternative Name

Tactile; T cell activation, increased late   expression; Cluster of Differentiation 96

UniProt

P40200

Immunogen

The original antibody was generated by   immunizing SJL white mice with various forms of the human CD96 extracellular region,   including versions tagged with Flag or mFc, as well as CHO cells   overexpressing human CD96.

Application Notes

In an ELISA assay, this antibody demonstrated an EC50 of 0.074 nM and 0.096 nM against human and cynomolgus   CD96, respectively. In another ELISA, this antibody demonstrated the ability   to specifically bind to CD96 overexpressing CHO-S cells with an EC50 of 0.036   nM. This antibody blocked the interaction between CD96 and CD155-expressing   CHO cells with an IC50 of 0.149nM. In an SPR assay, the antibody demonstrated   an affinity of 1.35 nM to Human CD96. A competitive ELISA assay demonstrated   that this antibody does not compete with Ab04854 Anti-CD96 [h1722-010],   indicating they target different epitopes. This antibody can enhance the   killing of tumor cells by NK cells.

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.