Key features and details | |
Cat. No. | MABL-1166 |
Name | Anti-CD98 mAbs |
Clone No. | AFD- 8-34B |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, in vivo, inhibition, SPR, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CD98. |
Alternative Name | LAT1; E16; MPE16; SLC7A5 |
UniProt | Q01650 |
Immunogen | The original antibody was generated by immunizing male 129S6/SvEv mice with human CD98 (huCD98)-expressing tumor cells. |
Application Notes | The murine version of the antibody inhibited tumor growth in Ramos xenografts and AML (OCI-AML-3) xenografts. Further, the chimeric version of the antibody was tested, confirming that also this version of the antibody could inhibit in vivo tumor growth with efficacy comparable to their parental counterparts. The antibody showed positive staining in 10/15 malignant melanomas and 4/18 lung cancers. In addition, the antibody gave positive staining in 6 of 6 frozen lung cancer samples and 2 of 2 frozen melanoma samples. Binding affinities of the murine and chimeric version of the antibody were determined using FACS analysis (Kd values between 0.9 nM and 4.5 nM). The chimeric antibody was also subjected to FACS analysis using three AML primary tumor samples and one cell line expressing cynomolgus monkey CD98 (cynCD98). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
