Key features and details | |
Cat. No. | MABL-1217 |
Name | Anti-CK18 (Cytokeratin 18) mAbs |
Clone No. | AFD- C-04 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | canine, Human |
Basic Information | |
Specificity | This antibody is specific for CK-18. |
Alternative Name | KRT18; K18; CK-18; Keratin-18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18 |
UniProt | P05783 |
Immunogen | The original antibody was raised by immunizing a BALB/c mouse with keratin-enriched preparations from cultured A431 cells. |
Application Notes | The original version of this antibody was used for immunohistochemistry on sections of teratoma derived from iPS cells (Shao et al., 2009; PMID:19238173), on rat endometrial cell sheets and the resected uteri (Kuramoto et al., 2018; PMID:29980256), on fresh samples from either tumours or normal tissues distant from tumours (Bártek et al., 1991; PMID:1716305), and on sebaceous epithelioma tissue from 2 dogs (Yoon and Park., 2016; PMID:27822240). To determine the specificity of this antibody immunofluorescence was performed on A431 cells and the HeLa cytoskeleton. Further a western blot was performed on filament-enriched preparations using this antibody (Bártek et al., 1991; PMID:1716305). This antibody was also used for immunofluorescence on Fresh IT specimens in a study on nose polyps (Yu et al., 2013; PMID:24033346). |
Antibody First Published | Bártek et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology J Pathol. 1991 Jul;164(3):215-24. PMID:1716305 |
Note on publication | The original paper describes A series of 14 new mouse monoclonal antibodies (MAbs) to keratins the data suggesting their potential value in the differential diagnosis of human tumours. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
