Anti-class I MHC mAbs

Cat. No.

MABL-1218

Name

Anti-class I MHC mAbs

Clone No.

AFD- 5H7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, IP, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for HLA class I   histocompatibility antigen all different chains. Antigen- presenting major   histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta   2 microglobulin displays primarily viral and tumor-derived peptides on   antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR)   on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell   immune response to eliminate infected or transformed cells.

Alternative Name

HLA class I histocompatibility antigen; HLA; Human leukocyte antigen

UniProt

P04439

Immunogen

BALB/c mice were immunized by a series of four   to six monthly injections of human PBLs, human Jurkat tumor ceIls, o  enriched T lymphocytes.

Application Notes

The biotinylated antibody was used for a   blocking experiment in human PBLs (Smith et al, 1994; pmid:7913109). Flow   cytometry was preformed on human PBMC using this antibody (Woodle et al,   1997; pmid:9036961). This antibody was used for immunoprecipitation on C1R   and T2. HMy2.C1R (C1R) are HLA-A negative and -B35 low human B-lymphoblastoid   cells, and 0.174 X CEMR (T2) are T-B lymphoblastoid hybrid cells that are   missing a large region of the MHC, including the genes encoding TAP1 and TAP2   (Dangoria et al, 2002; pmid:11978783).

Antibody First Published

Smith et al. Inhibition of T cell activation   by a monoclonal antibody reactive against the alpha 3 domain of human MHC   class I molecules J Immunol. 1994 Aug 1;153(3):1054-67. PMID:7913109

Note on publication

In this paper the antibody was generated by   immunizing mice with human PBLs, human jurkat tumor cells or enriched T   lymphocytes

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.