Key features and details | |
Cat. No. | MABL-1223 |
Name | Anti-Claudin-4 mAbs |
Clone No. | AFD- KM3900 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, neutralizing, therapeutic, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody specifically recognizes the conformational structure and binds to extracellular loop 2 of CLDN4. The antibody does not react with denatured CLDN4 protein. The antibody does not cross react with CLDN3, 5, 6, and 9. Claudin-4 is a tetraspanin transmembrane protein of tight junction structure and is highly expressed in pancreatic and ovarian cancers. |
Alternative Name | CLDN4; Claudin 4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein; FERM BP-10751 |
UniProt | O14493 |
Immunogen | The original antibody was generated from BXSB mice immunized with pancreatic cancer cells. |
Application Notes | Cell lysates were prepared from pancreatic cancer cells (Capan-2 and HPAF-II) and CLDN/CHO cells, and subjected to immunoprecipitation analysis. The antibody was shown to specifically precipitate CLDN4. This antibody detected CLDN4 expressed on CHO cells by flow cytometry. Furthermore, binding of the antibody was detected on CLDN4-expressing pancreatic and ovarian cancer cells, but not on negative cells. Moreover, the chimeric IgG1 (KM3934) version of the antibody was made and its antitumor efficacy was evaluated. KM3934 induced dose-dependent antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in vitro, and significantly inhibited tumor growth in MCAS or CFPAC-1 xenograft SCID mice in vivo (Suzuki et al., 2009; PMID:19555390). The antibody strongly bound to the CLDN4 expressed in breast cancer cells, colorectal cancer cells and prostate cancer cells. |
Antibody First Published | Suzuki et al. Therapeutic antitumor efficacy of monoclonal antibody against Claudin-4 for pancreatic and ovarian cancers Cancer Sci. 2009 Sep;100(9):1623-30. PMID:19555390 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
