Anti-CNN1 mAbs

Cat. No.

MABL-1229

Name

Anti-CNN1 mAbs

Clone No.

AFD- Mab420

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vitro, in vivo, SPR, ELISA, FC, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human and   cynomolgus monkey CNN1.

Alternative Name

IBP-10; Protein GIG1; Protein CYR61;   IGF-binding protein 10; IGFBP-10; CCN family member 1; Cysteine-rich   angiogenic inducer 61; Insulin-like growth factor-binding protein 10

UniProt

O00622

Immunogen

The original antibody was raised by   immunizing a BALB/c or NMRI mouse with recombinant human CCN1 containing the   single amino acid exchange E173D or F185L

Application Notes

The original antibody was used for flow   cytometry on suspension adapted HEK293 cells. In a further flow cytometry   experiment it was shown that the antibody also bound to cynomolgus monkey   CNN1 This antibody was used for an ELISA on both human and mouse CNN1. The   binding affinity of this antibody to human CNN1 was determined using surface   plasmon resonance. It showed a Kd of 3.02 * 10^-8. This antibody was used for   immunofluorescence on NIH 3T3 cells or suspension adapted HEK293 cells   transfected with the expression plasmids driving the expression of the CCN1.   It showed it could correctly label the CNN1 protein in vitro. This antibody   was used in vivo on different tumor cell lines xenografted into a SCID-beige   mice. It was effective for SKOV3 cells and Panc-1 cells but not MDA-MB-435   (US20120258112A1).

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.