Anti-Collagen alpha-1(VII) chain mAbs

Cat. No.

MABL-1232

Name

Anti-Collagen alpha-1(VII) chain   mAbs

Clone No.

AFD- LH7.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IHC

Species Reactivity

Bovine, Goat, Guinea Pig, Marmoset, Monkey,   Pig, Sheep, Human

Basic Information

Specificity

 This antibody is specific for amino acids   252 to 361 of Collagen alpha-1(VII) chain.

Alternative Name

 COL7A1; LC collagen; Long-chain collagen;   collagen VII

UniProt

 Q02388

Immunogen

The original antibody was raised by   immunizing a mouse with 1% Nonidet P40 extract of trypsinized cultured human   keratinocytes.

Application Notes

The original antibody was used for western   blotting on overlapping recombinant type VII collagen fusion proteins and   pepsinized human type VII (Lapiere et al., 1994; PMID:7530994), and on type   VII collagen fusion proteins (Tanaka et al., 1994; PMID:7524608). Further   this antibody was used for an ELISA on non-overlapping type VII collagen   fusion proteins encompassing both non- collagenous amino- and   carboxyl-terminal domains and the collagenous helical domain (Lapiere et al.,   1994; PMID:7530994). This antibody was used for immunohistochemistry on   normal adult human skin freshly obtained from two different sites (abdomen   and arm) (Shimizu et al., 1990; PMID:2354110), on different human tumours   (Wetzels et al., 1991; PMID:1867328), and on normal human tissue (Heagerty et   al., 1986; PMID:3527251).

Antibody First Published

Heagerty et al. Identification of an   epidermal basement membrane defect in recessive forms of dystrophic   epidermolysis bullosa by LH 7:2 monoclonal antibody: use in diagnosis Br J   Dermatol. 1986 Aug;115(2):125-31 PMID:3527251

Note on publication

Using indirect immunofluorescence the   original paper found intense labelling with LH 7:2 at the epidermal basement   membrane (EBM) of normal skin, and in skin samples from patients with   simplex, junctional, dominantly inherited dystrophic and acquired forms of   epidermolysis bullosa (EB), as well as bullous pemphigoid.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.