Key features and details | |
Cat. No. | MABL-1231 |
Name | Anti-Coat nucleoporin mAbs |
Clone No. | AFD-57 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Saccharomyces cerevisiae |
Basic Information | |
Specificity | The antibody interacts Seh1•Nup85•Nup145C1-123. |
Alternative Name | Nucleoporin NUP145; Nuclear pore protein NUP145; Nucleoporin NUP145N; N-NUP145; Nucleoporin NUP145C; C-NUP145; Nucleoporin NUP85; Nuclear pore protein NUP85 Nucleoporin NUP85; Nucleoporin SEH1; Nuclear pore protein SEH1; SEC13 homolog 1 |
UniProt | P49687; P46673; P53011 |
Immunogen | The original antibody was generated by panning against CNC subunits. |
Application Notes | The specificity of the antibody was tested against the assembled biotinylated yeast CNC, as well as individual biotinylated subunits using a single point competitive ELISA assay. |
Antibody First Published | Stuwe et al. Architecture of the nuclear pore complex coat. Science. 2015 Mar 6; 347(6226): 1148–1152. PMID:25745173 |
Note on publication | The paper describes the crystal structure of a reconstituted coat nucleoporin complex (CNC) from S. cerevisiae. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |