Anti-Collagen I mAbs

Cat. No.

MABL-1234

Name

Anti-Collagen I mAbs

Clone No.

AFD- AFA

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, therapeutic, Block

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human collagen  I, showing high affinity to the GDF sequence (in A2_DGDFY, part of the   α2(I)C-telopeptide), suggesting it is a critical epitope.

Alternative Name

CTTR1; α2Ct; α2(I)C-telopeptide; C-terminal   telopeptide of the α2 chain; Procollagen I

UniProt

Immunogen

The original antibody was produced by immunizing   mice with a synthetic peptide (GGGYDFGYDGDFYRA) whose sequence corresponds to   that of the C-terminal telopeptide of the α2 chain (α2Ct).

Application Notes

This antibody binds human collagen I and   blocks the binding activity of its C-terminal telopeptide region (denoted as   CTTR1) consisting of two α1(I)C-telopeptides (denoted as α1Ct) and one   α2(I)C-telopeptide (denoted as α2Ct). The KD of this antibody and its Fab and   scFv with native α2Ct present in procollagen I and with synthetic α2Ct were   663 pM, 268 nM, 21 pM, 57 nM, and 75nM for full-length AFA/procollagen I, Fab   AFA antibody/procollagen I, full-length AFA antibody/synthetic α2Ct, Fab AFA   antibody/synthetic α2Ct, and scFv/procollagen I, respectively   (US20200048333A1). A chimeric IgG version of the original IgA version of this   antibody was engineered (Fertala et al., 2013; PMID: 23586407). This antibody   has been shown to be an effective therapeutic tool for blocking collagen   fibril formation in various models (Fertala et al., 2017; PMID: 28972447)   (Fertala et al., 2017; PMID: 23586407).

Antibody First Published

Chung et al. Collagen fibril formation. A   new target to limit fibrosis J Biol Chem. 2008 Sep 19;283(38):25879-86. doi:   10.1074/jbc.M804272200 PMID:18650436

Note on publication

The original publication introduces a novel   concept for reducing fibrotic deposits by inhibiting the self-assembly of   collagen molecules into fibrils, utilizing monoclonal antibodies targeting   the telopeptide region to block collagen interactions and demonstrating   efficacy in reducing collagen fibrils in vitro and in organotypic constructs.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.