Anti-Collagenase type IV mAbs

Cat. No.

MABL-1242

Name

Anti-Collagenase type IV mAbs

Clone No.

AFD- 3G11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Immunoscintigraphy, in vivo, MTT assay, ELISA, IF, IHC

Species Reactivity

Mouse

Basic Information

Specificity

 This antibody is specific for type IV collagenase.

Alternative Name

 MMP2; MMP-2; MMP 2; Matrix metalloproteinase 2; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A

UniProt

 P33434

Immunogen

 The original antibody was generated by immunizing BALB/c mice with type IV collagenase.

Application Notes

The immunoreactivity of the original format of this antibody (mouse IgG1, κ) was measured via ELISA and was shown to decrease by approximately 10%-20% after cold iodination with 131- iodide. The   (131)I-labeled version of this antibody was demonstrated to be stable in   vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was   defined as a two-compartment model, with a 7.2 h T1/2α and a 345.2 h T1/2β,   and clear scintigraphic images of human lung carcinoma PG were obtained using   (125)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al.,   2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of   this antibody, and the resulting immunoconjugate maintained most of its   immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as   shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells   than free LDM by MTT assay.

Antibody First Published

Dai et al. [Immunoscintigraphy of anti-type IV collagenase monoclonal antibody in nude mice bearing human lung cancer  xenograft] Ai Zheng. 2003 Dec;22(12):1243-8.PMID:14693044

Note on publication

The original publication focuses on evaluating the tumor-specific distribution of the anti-type IV collagenase   monoclonal antibody (mAb) 3G11 through radio imaging in tumor-bearing nude   mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.