Anti-Complement C3 mAbs

Cat. No.

MABL-1243

Name

Anti-Complement C3 mAbs

Clone No.

AFD- 1H8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, FC, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human C3b. C3   plays a central role in the activation of the complement system. Its   processing by C3 convertase is the central reaction in both classical and   alternative complement pathways. After activation C3b can bind covalently,   via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Alternative Name

Complement C3; C3; C3 and PZP-like   alpha-2-macroglobulin domain- containing protein 1

UniProt

P01024

Immunogen

The mouse version of this antibody was raised by immunizing a mouse with human C3b and iC3b bound to a carbohydrate matrix via the alternative pathway of complement activation.

Application Notes

To show that a monoclonal antibody directed  against the merozoite and human polyclonal IgG from merozoite vaccine   recipients enhanced RBC invasion in a complement-dependent manner,   immunofluorescence was performed on merozoites with RBC using the mouse   version of this antibody (Biryukov et al, 2016; pmid:27333049). Flow   cytometry was performed on intact and lysed PNH erythrocytes using the mouse   version of this antibody to analyze the deposition of C3 (Lindorfer et al,   2010; pmid:20068220). While studying the role of the complement system in   childhood malaria, immunoprecipitation was performed on erythrocytes using   the mouse version of this antibody. Furthermore, a western blot was performed   on erythrocytes using this antibody (Pawluczkowycz et al, 2007; pmid:17911641). 

Antibody First Published

Beum et al. Complement activation and C3b deposition on rituximab- opsonized cells substantially blocks binding of   phycoerythrin-labeled anti-mouse IgG probes to rituximab J Immunol Methods. 2004 Nov;294(1-2):37-42. PMID:15604014

Note on publication

The ability of several commercially   available phycoerythrin (PE)-labeled anti-Mouse IgG antibodies to bind to B   cells opsonized in milieu which allow or preclude complement activation was   examined. Even when large amounts of fluorescently labeled RTX are bound to   the cells, binding of the anti- Mouse IgG probes is substantially inhibited   if C3b is deposited on the cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.