Key features and details | |
Cat. No. | MABL-1244 |
Name | Anti-Complement C3 mAbs |
Clone No. | AFD- KF7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA |
Species Reactivity | Guinea Pig |
Basic Information | |
Specificity | KF7 is highly specific for guinea pig C3, with no binding observed between KF7 and guinea pig C3a, rat C3 and human C3. |
Alternative Name | C3 |
UniProt | P12387 |
Immunogen | A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from bone marrow, a phage display library constructed and then used to produce soluble scFv fragments. |
Application Notes | ELISA was used to confirm the specificity of KF7 for guinea pig C3, with KF7 displaying high reactivity for gp C3. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed. KF7 has a higher signal intensity than MF9 and MA11 (Hawlisch et al, 2000). SPR was used to quantify the dissociation rate constant of KF7; KF7 has a dissociation rate constant of 1.6x10^-4 s^-1 (Hawlisch et al, 2000). |
Antibody First Published | Hawlisch et al, 2000. Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries J Immunol Methods. 2000 Mar 6;236(1-2):117- 31 PMID:10699585 |
Note on publication | Describes the original generation of KF7 and its characterisation through ELISA and SPR. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
