Anti-Complement C4d mAbs

Cat. No.

MABL-1252

Name

Anti-Complement C4d mAbs

Clone No.

AFD- LP69

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the C4d   protein.

Alternative Name

TDM16; C4A; Complement C4-B; C4B; C4B_2;   Basic complement C4; C4b- B; C4d-B; C4a anaphylatoxin; Complement C4 beta chain; Complement C4-B alpha chain; Complement C4 gamma chain; C3 and   PZP-like alpha-2-macroglobulin domain-containing protein 3; C4b-A

UniProt

P0C0L4; P0C0L5

Immunogen

The original antibody was raised by   immunizing a mouse with Complement C4d from normal human plasma.

Application Notes

The original version of this antibody was   used for flow cytometry on red blood cells from healthy donors and SARS-CoV-2   patients (Lam et al., 2021; PMID：34231390). The original version of this antibody was also used for a   western blot on Factor I and C4b-binding protein (Blom et al., 2016; PMID：27146825).

Antibody First Published

Blom et al. Testing the Activity of   Complement Convertases in Serum/Plasma for Diagnosis of C4NeF-Mediated C3   Glomerulonephritis J Clin Immunol. 2016 Jul;36(5)：517-27. PMID：27146825

Note on publication

The original paper studies the activity of   classical convertases directly in serum/plasma to screen samples from 13   patients with C3 glomerulopathies and identified one patient showing   significantly prolonged half-life of these enzymes.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.