Anti-Cortactin mAbs

Cat. No.

MABL-1260

Name

Anti-Cortactin mAbs

Clone No.

AFD- 4F11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF, IHC

Species Reactivity

Avian, Bovine, Hamster, Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for cortactin in   both birds and mammals. It recognizes 80 kDa and 85 kDa Cortactin in avian   cells, and also recognizes 80 kDa protein in rodent and human cells. Cortactin contributes to the organization of the actin cytoskeleton and cell  shape.

Alternative Name

Src substrate protein p85; CTTN1; Cortactin; p80

UniProt

Q01406

Immunogen

The mouse version of this antibody was   generated by immunizing mice with a mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src, and recognizes   Cortactin.

Application Notes

To characterise the binding of the mouse version of this protein to tyrosine- phosphorylated protein,   immunoblotting on tyrosine-phosphorylated proteins from CEFs, NIH 3T3, Rat-1,   HOS and 10W was performed. Furthermore, immunoprecipitation on the same samples was preformed. The antibody showed a reaction in both experiments and had   cross-reactivity with the other species (Kanner et al, 1990; pmid:2110361).   To confirm the specificity of the mouse version of this antibody,   immunoprecipitation of cortactin was preformed using this antibody.   Furthermore, the immunoprecipitated antibodies were western blotted with this antibody. The western blot showed bands corresponding to cortactin. Finally,   invadopodia were immunostained for cortactin using this antibody and showed   co- localization with antibody clone #170-10, which is also an anti-cortactin   antibody (Bowden et al, 1999; pmid:10442635). 

Antibody First Published

Kanner et al. Monoclonal antibodies   to individual tyrosine-phosphorylated protein substrates of oncogene-encoded   tyrosine kinases Proc Natl Acad Sci U S A. 1990 May;87(9):3328-32. PMID:2110361

Note on publication

To study transformation-relevant   tyrosine kinase substrates, we have generated monoclonal antibodies to   individual tyrosine phosphoproteins, including p130, p120, p110, and five   additional phosphoproteins (p210, p125, p118, p85, and p185/p64). These antibodies   detected several of the same tyrosine phosphoproteins in chicken embryo   fibroblasts transformed by avian retroviruses Y73 and CT10, encoding the yes   and crk oncogenes, respectively.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.