Key features and details | |
Cat. No. | MABL-1261 |
Name | Anti-Cortisol mAbs |
Clone No. | AFD- 5A4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | equilibrium dialysis, RIA, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds cortisol. It has also shown cross-reactivity to dexamethasone and prednisolone. This is due to a hydroxyl group bound to C11. Cortisol is a steroid hormone, in the glucocorticoid class of hormones. It is produced in many animals, mainly by the zona fasciculata of the adrenal cortex in the adrenal gland. It is produced in other tissues in lower quantities. It is released with a diurnal cycle and its release is increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation. This antibody has not shown any affinity for cortisol-21-hemisuccinate-BSA. |
Alternative Name | Hydrocortisone; ChEBI：17650 |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with cortisol-7-CM, followed by cortisol-3-CMO. Both forms of cortisol were bound to BSA. |
Application Notes | The specificity and affinity of this mouse version of this antibody was determined using equilibrium dialysis in dialysis cells fitted with a cellulose membrane of 3500 MW cutoff (Calvez et al, 1995; pmid：7898495). Further a radioimmunoassay was performed on I125-cortisol using the scFv version of this antibody (Calvez et al, 1995; pmid：7898495). The specificity of this antibody towards cortisol-3-CMO-BSA, cortisol, prednisolone and dexamethasone was also tested using equilibrium dialysis. ELISA was used to show that this clone was able to bind steroids conjugated to BSA through nonspecific hydrophobic interactions (PMID： 9820517). |
Antibody First Published | Calvez et al. Paratope characterization by structural modelling of two anti- cortisol single-chain variable fragments produced in E. coli. Mol Immunol. 1995 Feb;32(3): 185-98.PMID：7898495 |
Note on publication | Two monoclonal antibodies (mAbs), 5A4 and 6D6, directed against cortisol, have been obtained; 6D6 is used in an assay kit for cortisol. The antibodies also recognize other, structurally related steroids present in the sample assayed. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
