Key features and details | |
Cat. No. | MABL-1262 |
Name | Anti-Cotinine mAbs |
Clone No. | AFD- CT#45 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds cotinine. Cotinine is an alkaloid found in tobacco and is also the predominant metabolite of nicotine. It is used as a biomarker for exposure to tobacco smoke. |
Alternative Name | 486-56-6; ChEBI：68641; (S)-Cotinine; Cotinina; Cotininum |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a cotinine–albumin conjugate. |
Application Notes | This antibody was used in the development of an ELISA for determining urinary cotinine to monitor tobacco smoke exposure. This ELISA produced dose–response curves for cotinine ranging from 0.40–100 ng per assay and was able to discriminate nicotine, cotinine N-oxide, cotinine N- glucuronide (cross-reactivity was each 0.5%, taking cotinine as 100%), (3′R, 5′S)-3′-hydroxycotinine O- glucuronide (0.6%) and (R, S)-norcotinine (1.5%). Another major nicotine metabolite, (3′R, 5′S)-3′- hydroxycotinine, showed 8.4% cross-reactivity with this antibody (Kobayashi, 2011) |
Antibody First Published | Kobayashi et al. A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure. Analytical Methods 2011, Issue 9. |
Note on publication | Describes the use of this antibody to develop an ELISA to detect urinary cotinine. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
