Anti-CpaF mAbs

Cat. No.

MABL-1265

Name

Anti-CpaF mAbs

Clone No.

AFD-556

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Liberibacter asiaticus

Basic Information

Specificity

 Clone 556 was shown to bind purified CpaF by   ELISA (Yuan et al, 2015).

Alternative Name

 Component of type IV pilus

UniProt

 C6XFP4

Immunogen

BALB/c mice were immunised by injection of a high titre of Liberibacter asiaticus obtained from infected Diaphorina citri psyllids.   The spleens of immunised mice were removed and homogenised for RNA extraction   and cDNA synthesis.

Application Notes

Clone 556 was tested for Liberibacter asiaticus recognition as a phage-expressing scFv using blotting (Yuan et al,   2015). This format was also tested against specific recognition of the   antigen CpaF using ELISA.

Antibody First Published

Yuan et al. Development of single chain   variable fragment (scFv) antibodies against surface proteins of 'Ca.   Liberibacter asiaticus'. J Microbiol Methods. 2016 Mar;122：1-7.PMID：26744234

Note on publication

Describes the generation and brief   characterisation of anti-Liberibacter asiaticus scFv-formatted antibody   fragments specific for different antigens.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.