Key features and details | |
Cat. No. | MABL-1266 |
Name | Anti-CPS-500 mAbs |
Clone No. | AFD-18.44 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | electron microscopy, therapeutic, WB, ELISA, IF |
Species Reactivity | Cryptosporidium parvum |
Basic Information | |
Specificity | This antibody binds the CPS-500 antigen of sporozoites and merozoites of Cryptosporidium parvum. Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences. |
Alternative Name | β-mannosylated glycolipid, CPS-500 antigen |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with whole cells of Cryptosporidium parvum. |
Application Notes | This antibody reacts with both sporozoites and merozoites of C. parvum in an indirect immunofluorescence assay. This antibody is capable of neutralizing sporozoites and merozoites in a time dependent manner (PMID： 2193057; 2473130). This antibody can be used for the identification of sporozoites, merozoites and CPS-500 antigen of C. parvum using western blot. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID： 20086143). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID： 20086143). |
Antibody First Published | Bjorneby et al. Cryptosporidium parvum merozoites share neutralization- sensitive epitopes with sporozoites. J Immunol. 1990 Jul 1;145(1)：298-304. PMID：2193057 |
Note on publication | Describes the use of antibodies to determine common epitopes shared between merozoites and sporozoites of C. parvum. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
