Key features and details | |
Cat. No. | MABL-1267 |
Name | Anti-CR1 mAbs |
Clone No. | AFD- E11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Western blot, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | E11 is reactive to neutrophilic granulocytes and eosinophils; monocytes; macrophages; lymphocytes; erythrocytes; dendritic reticulum cells; tonsil tissue section; spleen tissue section; lymph node tissue section; gut tissue section; kidney podocytes and kidney tissue sections; chronic lymphocytic leukemia; acute myelomonocytic leukemia; acute monocyte leukemia; B cell lines (JY, MST, BR17); EBV- infected B cell cultures; myeloid cell lines (U937, HL60, THP 1-0). |
Alternative Name | CD35; Type 1 complement receptor; C3b/C4b receptor |
UniProt | P17927 |
Immunogen | BALB/c mice were immunised with cells from acute monocytic leukemia patients. |
Application Notes | E11 fluorescence staining of normal blood cells was detectable on 90% of monocytes, 95% of neutrophils, and 14%-24% of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013). |
Antibody First Published | Hogg et al, 1984. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3)：236-43. PMID：6368248 |
Note on publication | Describes the original production of the antibody and its characterisation through immunofluorescence. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
