Key features and details | |
Cat. No. | MABL-1308 |
Name | Anti-CXCR4 mAbs |
Clone No. | AFD- 414H5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, in vitro, in vivo, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CXCR4. |
Alternative Name | CD184; CXC-R4; CXCR-4; FB22; LAP-3; Fusin; HM89; LCR1; LESTR 1; NPYRL; C-X-C chemokine receptor type 4; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; Stromal cell-derived factor 1 receptor; SDF-1 receptor |
UniProt | P61073 |
Immunogen | The original antibody was generated by immunizing Balb/C mice with recombinant NIH3T3- CXCR4 cells and/or peptides corresponding to CXCR4 extracellular N-term and loops. |
Application Notes | The specificity of the antibody was confirmed by ELISA analysis. The original and the human chimeric formats of the antibody bound specifically to the human CXCR4-NIH3T3 transfected cell line but could not recognize the parent NIH3T3 cells by FACS analysis. The antibody's original format also recognized MDA-MB-231 breast cancer cells, U937 promyelocytic cancer cells, and Hela cervix cancer cells. The antibody's original and human chimeric formats competed for binding of SDF-1 to human CXCR4 receptor, expressed on CHO-K1 cells (% inhibition: 30±5% and 21±10% respectively). The original and human chimeric formats of the antibody could modulate the [35S]GTPγS binding at cellular membranes expressing CXCR4 receptor expressed on HeLa and NIH3T3/CXCR4 cell membranes (IC50 in NIH3T3/CXCR4 cells: 1.6 nM, 1.1 nM and in Hela cells 0.5 nM, 0.3 nM, respectively). Further, the antagonist potency was determined to be 51 nM in both cell lines. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
