Anti-Cyclosporin A mAbs

Cat. No.

MABL-1313

Name

Anti-Cyclosporin A mAbs

Clone No.

AFD- R454511

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallography, ELISA

Species Reactivity

n/a

Basic Information

Specificity

R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA.

Alternative Name

CsA; ciclosporin A, cyclosporine A

UniProt

Immunogen

BALB/c mice were immunised with a D-Lys^8-Cs   derivative coupled to a carrier protein.

Application Notes

Mutagenesis of Cs-derivatives identified   that R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA (Quesniaux et   al, 1990). In ELISA experimenets, R454511 was found to bind to Cs conjugated with both Thr^2 (Thr^2-Cs-BSA) and Lys^8 (D-Lys^8-Cs-BSA) (Quesniaux et al, 1990). The crystal structure of R454511 in complex with CsA has been obtained   to 2.65 Å resolution (Altschuh et al, 1992).

Antibody First Published

Quesniaux et al, 1990. Monoclonal antibodies   to cyclosporin are representative of the major antibody populations present in antisera of immunized mice. Mol Immunol. 1990 Mar;27(3):227-36.   PMID:2342487

Note on publication

Describes the original generation of the antibody and its characterisation through ELISA.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.