Key features and details | |
Cat. No. | MABL-1320 |
Name | Anti-Cytochrome b-245 light chain mAbs |
Clone No. | AFD- NS5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognises Cytochrome b-245 light chain (Epitope LLEYPRG+VKLFGP). |
Alternative Name | CYBA; flavocytochrome b; Cyt b; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox |
UniProt | P13498 |
Immunogen | |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected p22phox by western blot analysis. Further, the antibody immunoprecipitated Cyt b. The antibody showed moderate levels of inhibition of superoxide production by the NADPH oxidase complex (Taylor et al., 2004; PMID: 15585859). |
Antibody First Published | Taylor et al. Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit J Immunol. 2004 Dec 15;173(12): 7349-57. PMID: 15585859 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
