Key features and details | |
Cat. No. | MABL-1319 |
Name | Anti-Cytochrome b-245 light chain mAbs |
Clone No. | AFD-44.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognises Cytochrome b-245 light chain (Epitope ATAGRF+GGPQVNPI). |
Alternative Name | CYBA; flavocytochrome b; Cyt b; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox |
UniProt | P13498 |
Immunogen | |
Application Notes | The expression of p22phox from HEK-293 cells was confirmed by western blot with the antibody (Nakano et al., 2007; PMID: 17140397). The antibody detected p22phox by western blot analysis (Burritt et al., 1995; PMID: 7622517 and Taylor et al., 2004; PMID: 15585859). The antibody bound permeabilized neutrophils as shown by flow cytometric analysis. The antibody immunoprecipitated cytochrome b (Burritt et al., 1995; PMID: 7622517). |
Antibody First Published | Taylor et al. Cloning, sequence analysis and confirmation of derived gene sequences for three epitope-mapped monoclonal antibodies against human phagocyte flavocytochrome b. Mol Immunol. 2007 Jan;44(4): 625-37. PMID:16563510 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
