Key features and details | |
Cat. No. | MABL-1326 |
Name | Anti-Cytokeratin 10 mAbs |
Clone No. | AFD- RKSE60 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IHC |
Species Reactivity | canine, Rat, Swine, Zebrafish, Human, Mouse |
Basic Information | |
Specificity | The original antibody reacts exclusively with Cytokeratin 10 which is present in Keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. |
Alternative Name | K10; CK-10; Keratin K10; Keratin type I cytoskeletal 10; Cytokeratin-10; Keratin-10 |
UniProt | P13645 |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with Cytokeratins from the Human epidermis. |
Application Notes | The original antibody specifically reacted with keratinizing stratified epithelia and squamous cell carcinomas derived from these by IHC (Ramaekers et al. 1983; PMID： 6350235). The antibody stained cytokeratin 10 from cultured human keratinocytes by immunohistochemistry (Muijen et al, 1987; PMID： 2442018). This antibody was used for detection of cytokeratin 10 in psoriatic skin and normal skin by flow cytometry (van Erp et al., 1989; PMID： 2479273). The antibody detected cytokeratin 10 from human foreskin keratin preparation, by western blot analysis (Ramaekers et al., 1990; PMID: 1690512). |
Antibody First Published | Ramaekers et al. Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumors: an overview Histochem J. 1983 Jul;15(7)：691-713. PMID：6350235 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
