Key features and details | |
Cat. No. | MABL-1363 |
Name | Anti-demetalated SOD1 mAbs |
Clone No. | AFD- D3-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognized SOD1A4V, SOD1H46R, SOD1G85R, SOD1G93A, and SOD1I112M, but not SOD1WT. Further the antibody did not recognize SOD1 in patients with sporadic ALS or other neurodegenerative diseases. |
Alternative Name | SOD1; SOD1G93A |
UniProt | |
Immunogen | The original antibody was raised by immunizing a C57Bl/6 mouse with the antigen peptide SOD1G93A. |
Application Notes | The original antibody was used for immunohistochemistry on spinal cord sections from patients with FALS. This antibody was used in vivo on transgenic rats with SOD1H46R mutation. The treatment improved phenotypes and extended the lifespan of the rats. This antibody was used for an ELISA on recombinant protein SOD1G93A (Minamiyama et al., 2023; PMID:36874245). |
Antibody First Published | Minamiyama et al. Efficacy of oligodendrocyte precursor cells as delivery vehicles for single-chain variable fragment to misfolded SOD1 in ALS rat model Mol Ther Methods Clin Dev. 2023 Feb 4:28:312-329. PMID: 36874245 |
Note on publication | In the original paper using a Borna disease virus vector that is pharmacologically removable and episomally replicable in the recipient cells, we successfully transformed wild-type OPCs to secrete scFv of a novel monoclonal antibody (D3-1), specific for misfolded SOD1. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
