Key features and details | |
Cat. No. | MABL-1371 |
Name | Anti-Desmin mAbs |
Clone No. | AFD- RD301 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IHC |
Species Reactivity | canine, Chicken, Hamster, Rabbit, Rat, Swine, Zebrafish, Human, Mouse |
Basic Information | |
Specificity | The antibody is specific for desmin. Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). |
Alternative Name | |
UniProt | P17661 |
Immunogen | The original antibody was generated by immunizing a mouse with a cytoskeletal extract of Chicken gizzard. |
Application Notes | The antibody detected desmin in western blot analysis. The original antibody showed staining pattern of desmin in BHK cells (Quax et al., 1985; PMID: 3855248). The antibody stained desmin on smooth muscle cells of rat prostatic tissue by indirect immunofluorescence (Verhagen et al., 1988; PMID: 2458583). |
Antibody First Published | Quax et al. Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer Cell. 1985 Nov;43(1): 327-38. PMID: 3855248 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
