Key features and details | |
Cat. No. | MABL-1384 |
Name | Anti-Digoxigenin A mAbs |
Clone No. | AFD- Clone 3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for digoxigenin A. Digoxigenin (DIG) is a hydroxy steroid that consists of 5beta-cardanolide, found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata. It is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications. |
Alternative Name | Digoxigenin; DigA; DOG; ChEBI: 42098; CAS:1672-46-4 |
UniProt | |
Immunogen | The original antibody was isolated from a IgM-derived scFv phage antibody library created from the peripheral blood lymphocytes of 20 non-immunized doners. The specific clone was isolated by panning the library against digoxigenin. |
Application Notes | The binding specificity of this antibody for digoxigenin was checked using ELISA. This antibody did not bind digoxigenin coupled by a methylcarbonyl (Dig-Met) or succinyl (Dig-Suc) type linker to BSA (PMID: 9305722). |
Antibody First Published | Dörsam et al. Antibodies to steroids from a small human naive IgM library. FEBS Lett. 1997 Sep 1;414(1):7-13. PMID: 9305722 |
Note on publication | Describes the generation of antibodies against steroids from a human naive IgM library. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
