Key features and details | |
Cat. No. | MABL-1396 |
Name | Anti-Disialoganglioside 2 mAbs |
Clone No. | AFD- 3G6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, ELISA, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the GD2 antigen, but does not cross react with GD3, GT1b, GD1b, GD1a, GM1, GM3 and GM4. |
Alternative Name | GD2 |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human neuroblastoma cells. |
Application Notes | The binding studies of this antibody to various human tissues was done using ELISA and indirect immunofluorescence. This antibody also showed comparable reciprocal cross-blocking in radioimmunoassay on LAN-1 NB cells or NB glycolipid extracts (PMID: 2580625). This antibody is also recommended for the study of distribution of the GD2 antigen on various types of cancer tissues. This antibodhy was also used in the development of a chimeric receptor 3G6-CD28, which provides CD28 signaling upon specific recognition of the GD2 antigen on tumor cells (PMID: 9705944). Expression of GD2 expressing cells was done by flow cytometry using 3G6. This antibody was also used in anti-GD2 therapy to test the survival and metastasis after challenge with EL4 cells (PMID: 16288049). |
Antibody First Published | Cheung et al. Monoclonal antibodies to a glycolipid antigen on human neuroblastoma cells. Cancer Res. (1985) Jun;45(6):2642-9. PMID:2580625 |
Note on publication | Describes the generation of five antibodies against a human neuroblastoma cell surface glycolipid antigen. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
